PEP4-Allele Modification Provides an Industrial Brewing Yeast with Malate Stress Tolerance

Acid stress is a challenging condition that yeast cells must overcome during fermentation. Enhancing the inherent tolerance of industrial Saccharomyces cerevisiae to organic acid stresses crucial for increasing fermentation efficiency and reducing economic costs. In previous study, we constructed strain SWY85S with improved citric by modifying second PEP4-allele. Malic dominant in grapefruit, which forms acidic constituents wine mash finished products. We investigated malic comparison one PEP4-allele disrupted wild-type this study. Our results revealed demonstrated greater stress, regardless whether it was cultured adequate nutrient supplies or under amino starvation. Moreover, performed remarkably converting glucose ethanol stress. This study provides insights into role vacuolar coding product response environmental physiological mechanism withstand